Flamingo Land bosses have submitted fresh plans to create a “tourist resort” with more than 200 rooms and a monorail on the shores of Loch Lomond.
The Yorkshire-based firm published its application to build the £40m destination after making “fundamental changes” to its original plans.
The resort would include a 60-bed hotel, 127 self-catering lodges, a leisure pool and water park facility and a monorail, as well as restaurants, cafes and retail areas.
However the plans face renewed opposition over concerns about scale and impact on the land.
More than two years ago, Flamingo Land was forced to withdraw its original bid following a campaign led by the Green party.
The appeal saw 50,000 objections lodged with the Loch Lomond National Trossachs Park whose officers recommended the application not be granted.
Following the news of Flamingo Land’s new bid, Greens asked residents to once again sign their petition to stop the development of apartments, lodges and a budget hotel.
The online campaign states: “Their new application has only just been submitted and there is a huge amount of information for local residents and our campaign team to digest.
“What’s already clear is that their plans are once again far too big, inappropriate for the location and will involve handing over a special piece of publicly owned land for the sake of private profit.”
The party also say they will provide support for anyone wishing to comment on the application ahead of the National Park’s planning committee making its decision.
Changes to the earlier rejected proposals include a revised vision of the site adjacent to the pierhead, separate to the public slipway managed by the National Park, and the complete removal of any development in the ancient woodland of Drumkinnon Wood.
The application will be determined by National Park Authority in due course.