Industry leaders say loss of coach firms could jeopardise tourism in the region.
21 road accidents resulting in injury were recorded between Tyndrum and Lix Toll.
Construction of the £207m line to Newhaven is scheduled to last until summer 2021.
Councillors approve plan to completely transform Glasgow's civic centre.
Industry leaders believe buses can be part of the solution with a shift from private cars to sustainable transport.
The limit will be introduced to residential streets, the city centre and where there are high levels of walking or cycling.