The First Minister has told the PM that the £20bn would be better spent in Scotland and Northern Ireland.
Boris Johnson accused of 'personal animosity' towards Nicola Sturgeon as he hints at blocking FM's attendance.
Britain officially split from the European Union at 11pm, three-and-a-half years since the Brexit vote.
After 11pm, Britain will immediately enter a transition arrangement with the EU, keeping it aligned with Brussels.
The First Minister said a ballot could be held without Westminster's consent provided it's ruled as legal.
More than half of Scots would vote for independence - but they don't support a referendum this year.
Immigration is reserved to Westminster but the Scottish Government is calling for powers to be split.
Some MPs cheered as royal assent was confirmed for the European Union (Withdrawal Agreement) Act.
The Welsh Assembly joined Holyrood and Stormont in formally refusing consent to the Bill.
The First Minister's spokesman said she remains 'committed' to holding a vote this year.
MSPs were told by the UK Government medicine pricing would not be part of post-Brexit negotiations.
The Prime Minister said a new independence vote would cause continued 'political stagnation'.
The European Union (Withdrawal Agreement) Bill was passed by 330 votes to 231.
Boris Johnson told MPs during Prime Minister's Questions that the people of Scotland "voted decisively" in 2014 to stay in the UK in a "once in a generation choice".
Annie Wells MSP has called on the PM to hold a summit on the issue 'as soon as possible'.
Ex-justice secretary Kenny MacAskill - and newly-elected MP - said a longer wait is 'no bad thing'.
Data released to the Scottish Liberal Democrats under freedom of information legislation has been branded 'miserable'.
It will recommend Holyrood does not consent to the UK Government's Withdrawal Agreement Bill.
As Boris Johnson's government lays out its legislative agenda, it dismisses a 2020 indyref as a 'damaging distraction'.
The First Minister has called for the Prime Minister to 'agree and negotiate' the transfer of powers to Holyrood to hold indyref2.