Teams in the Scottish Championship, League One and League Two can return to training next week.
Contact training can resume from Monday in line with government guidance, following an update from the joint response group (JRG).
The move applies to both the youth and adult game and also includes the professional and non-professional game.
The details were outlined to Championship, League One and League Two clubs on Friday.
Rod Petrie, chair of the JRG, said: “This is a significant and hugely positive step forward for the whole of Scottish football and means that training can resume for both the professional and grassroots game from Monday.
“I understand the sacrifices players, coaches and clubs have been forced to make to get to this point but the health of the nation remains our absolute priority.
“Through the JRG and its grassroots sub group we have worked hard since yesterday’s announcement from the First Minister to put the latest government advice into context for the professional and non-professional game and to make the message as clear and as simple as possible.
“Training can resume on Monday in line with government’s ongoing social distancing measures and advice and, if the virus continues to be contained in Scotland, then the path to competitive football matches being played by October is tangible.”
Neil Doncaster, SPFL chief executive, said: “This is a major milestone in getting our game fully up and running again. It’s hard to overstate the significance of this development for clubs outwith the Premiership, which can now start preparing with confidence for the Betfred Cup on October 6.
“The next crucial step will be allowing Scottish football fans back into games, based on the comprehensive range of safety measures which have been developed by clubs in close consultation with health experts.
“Discussions on pilot events at Premiership matches are now under way and everyone is determined, for the sake of the Scottish game as a whole, to ensure these go smoothly and safely.”