A “limited number” of fans will be allowed into Murrayfield for Edinburgh v Glasgow Warriors next Friday.
Scottish Rugby said it has been given the green light to host supporters at the match in the capital.
It will be the first professional rugby match in the United Kingdom to welcome spectators since the country went into lockdown.
Dominic McKay, Scottish Rugby’s chief operating officers, said: “Scottish Rugby is delighted that a limited number of spectators will be in BT Murrayfield on Friday 28th August to watch the Guinness PRO14 match between Edinburgh Rugby and Glasgow Warriors.
“We are pleased our work with the Scottish Government will ensure that players, support staff and fans will be covered by a comprehensive event plan for everyone’s safety including physical distancing requirements.
“Scottish Rugby would like to thank government ministers and officials who have helped us make this happen, and our own colleagues who pulled together a robust operational plan for the match.
“We hope that our experience and learnings from a live spectator event next Friday can help all of Scottish sport, and the wider events industry restart.”
The fixture is designated as an Edinburgh Rugby home match. Ticketing information will be issued by the club early next week.