Physical distancing on trains and at rail stations across Scotland will be reduced to a one-metre minimum from Monday.
ScotRail and Network Rail announced the change as part of the latest Scottish Government guidance.
Capacity on trains will remain “significantly reduced” with people also urged to consider alternative options where possible.
Face coverings became mandatory on Scottish public transport on Monday June 22 with ScotRail handing out more than 20,000 masks at a dozen stations.
David Simpson, ScotRail operations director, said: “We’ve worked closely with staff, trade union colleagues, and the government to satisfy ourselves that we have sufficient safety measures in place to move to a minimum of one metre physical distancing on trains and at stations.
“The provision of face masks and hand sanitisers at our busiest stations are just some of the measures we introduced in recent weeks to keep staff and customers safe.
“But we need everybody to continue taking personal responsibility for their travel choices, because physical distancing can’t be guaranteed.
“Customers who do travel with ScotRail should follow our five rules for safer travel, including the wearing of a face covering on trains and at stations.”