Kate and Gerry McCann have released a new image of what their missing daughter Madeleine could look like now.

The picture has been issued ahead of the second anniversary of her disappearance. It shows what experts think Madeleine McCann might look like aged six.

Glasgow-born Mr McCann and his wife have also spoken to US television talk show host Oprah Winfrey about the hunt for their daughter.

Madeleine disappeared, almost aged four, while on holiday with her parents and siblings in Portugal.

The McCanns at first found it difficult to look at the new photograph.

The couple’s spokesperson Clarence Mitchell explained: “Kate has said that she found it very difficult. In fact, it’s true that at first she didn’t quite recognise her daughter. But she’s absolutely adamant that if Madeleine were to walk past her today, she would of course recognise her in the flesh.

“It does take some getting used to. She remembers Madeline two years ago. You realise how much a child’s face can change.”

As part of their international campaign to raise awareness of their daughter’s disappearance, the McCanns appeared on Oprah Winfrey’s popular show.

However, after investigating Portuguese police files, a former detective believes Madeleine walked out of her family’s holiday apartment and was snatched by an opportunist.

Criminologist Mark Williams-Thomas said: “On the morning she spoke to Gerry and Kate: ‘The twins woke up last night’. They reassured her and told her: ‘Yes, we’re only in a tapas bar down the road’.

“So I think what happened, on that evening she woke up, she placed neatly her teddy bears, she realigned the bed, she wandered out of the apartment, through the unlocked patio door, out onto the outside, and that was where she was abducted.”

Mrs McCann told Oprah that she goes into Madeleine's bedroom at least twice a day.

She hopes the latest appeal will finally help to find her daughter.