Killer Luke Mitchell's fight against his conviction will not be heard by the UK's highest court.
On Wednesday, three Supreme Court justices refused permission for Mitchell, 23, to take his appeal to the London court.
Mitchell was ordered to serve at least 20 years for the 2003 murder of his 14-year-old girlfriend Jodi Jones in Dalkeith, Midlothian.
He applied directly to the court to hear his case after judges in Scotland did not grant him leave to take his case further.
But the Supreme Court justices have refused to hear the case, saying Mitchell's appeal against conviction is "closed".
Mitchell, who was 14 at the time of the murder, has always protested his innocence but his original appeal against conviction was rejected by senior judges in Scotland in 2008.
It is believed Mitchell had hoped a fresh appeal would be heard by the Supreme Court in light of a high-profile human rights decision it gave in 2010. The Cadder ruling put an end to police being able to question suspects without the option of legal representation.
However, it is understood that Mitchell's Supreme Court bid was refused because his initial appeal against conviction had been dealt with before the Cadder ruling was issued and it could not therefore be reopened.
Jodi was murdered on June 30 2003. She had been stripped, tied up and stabbed to death and her mutilated body dumped in woods near her home.
Mitchell was jailed for life in 2005. He was convicted of the killing following what was at the time the longest single-accused murder trial in Scottish legal history.
Since his conviction appeal failed, his subsequent attempts to have additional grounds of appeal heard by judges in Scotland have been refused and his attempt to have his minimum jail term cut also failed.
At the various stages of the case, Mitchell's legal team have raised questions about the way police interviewed the then 15-year-old boy and about the Crown's use of identification evidence during his high-profile trial at the High Court in Edinburgh.
It is unclear whether Mitchell will have a chance to take his case any further to the European Court of Human Rights in Strasbourg.